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Reckleen_1_Cas9
(Plasmid #233456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233456 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Custom built using Marburg Collection
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Level 2 RECKLEEN plasmid
  • Alt name
    Ptac lambda Red operon, Ptet cas9, J23117 acrIIA4, Ptet sfgfp dropout sgRNA
  • Alt name
    pMB1 ori, and ApmR (aac(3)-IV)
  • Species
    Other

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Reckleen_1_Cas9 was a gift from Anke Becker (Addgene plasmid # 233456 ; http://n2t.net/addgene:233456 ; RRID:Addgene_233456)

  • For your References section:

    RECKLEEN: a lambda Red/CRISPR-Cas9 based single plasmid platform for fast, efficient, markerless, and scarless genome editing in Klebsiella pneumoniae. Elsayed E, Stukenberg D, Schmeck B, Becker A. Research Square (2025) [Preprint]. doi: https://doi.org/10.21203/rs.3.rs-5859838/v1. 10.21203/rs.3.rs-5859838/v1
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