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Reckleen_3_multieditdropout
(Plasmid #233458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233458 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Custom built using Marburg Collection
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
  • Alt name
    sfgfp dropout for multitarget editing in positions 4 & 5 using RECKLEEN_3plus_sgRNA helper plasmids
  • Alt name
    pMB1 ori, and ApmR (aac(3)-IV)
  • Species
    Other

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Reckleen_3_multieditdropout was a gift from Anke Becker (Addgene plasmid # 233458 ; http://n2t.net/addgene:233458 ; RRID:Addgene_233458)

  • For your References section:

    RECKLEEN: a lambda Red/CRISPR-Cas9 based single plasmid platform for fast, efficient, markerless, and scarless genome editing in Klebsiella pneumoniae. Elsayed E, Stukenberg D, Schmeck B, Becker A. Research Square (2025) [Preprint]. doi: https://doi.org/10.21203/rs.3.rs-5859838/v1. 10.21203/rs.3.rs-5859838/v1
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