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Addgene

pMTKM31
(Plasmid #233503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Backbone manufacturer
    John Dueber, Addgene plasmid #65108
  • Backbone size w/o insert (bp) 2676
  • Vector type
    Yeast Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGK1-tetO2p
  • Species
    K.marxianus Y-1190
  • Mutation
    addition of two tetracycline operator sequeneces

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.03.636257 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMTKM31 was a gift from Vincent Martin (Addgene plasmid # 233503 ; http://n2t.net/addgene:233503 ; RRID:Addgene_233503)

  • For your References section:

    Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a Host for Lactose Valorization. Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, Martin VJJ. ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096. 10.1021/acssynbio.5c00096 PubMed 40629255
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