[#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient
(Plasmid
#233506)
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PurposeLentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 233506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti CMV/TO Zeo DEST (644-1)
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Backbone manufacturerModifed from CMV-loxp-DsRed-loxp-eGFP, Daniele Gilkes, Addgene #141148
- Total vector size (bp) 8260
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Blasticidin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsRed loxp eGFP
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Nanopore (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPlasmid derived from Addgene #141148 and #99610
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
[#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient was a gift from Simon Grelet (Addgene plasmid # 233506 ; http://n2t.net/addgene:233506 ; RRID:Addgene_233506) -
For your References section:
Nerve-to-cancer transfers of mitochondria during cancer metastasis. Hoover G, Gilbert S, Curley O, Obellianne C, Lin MT, Hixson W, Pierce TW, Andrews JF, Alexeyev MF, Ding Y, Bu P, Behbod F, Medina D, Chang JT, Ayala G, Grelet S.. Nature. 2025; In Press.