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Addgene

p300 KAT LIC 1M
(Plasmid #233587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 MBP TEV LIC cloning vector (1M)
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6462
  • Total vector size (bp) 7620
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p300 KAT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1158
  • GenBank ID
    NC_000022.11
  • Entrez Gene
    EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis MBP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p300 KAT LIC 1M was a gift from Scott Showalter (Addgene plasmid # 233587 ; http://n2t.net/addgene:233587 ; RRID:Addgene_233587)

  • For your References section:

    Probing Enzymatic Acetylation Events in Real Time With NMR Spectroscopy: Insights Into Acyl-Cofactor Dependent p300 Modification of Histone H4. Dewing SM, Showalter SA. Proteins. 2025 Jun 1. doi: 10.1002/prot.26848. 10.1002/prot.26848 PubMed 40452297
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