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Addgene

pLentiCRISPR-v1-sgDHFR
(Plasmid #233898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiCRISPR v1
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting DHFR
  • gRNA/shRNA sequence
    CCCGGCAGATACCTGAGCGG
  • Species
    H. sapiens (human)
  • Entrez Gene
    DHFR (a.k.a. DHFR1, DHFRP1, DYR)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer GTTTTAAAATGGACTATCATATGCTTACCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiCRISPR-v1-sgDHFR was a gift from Jason Cantor (Addgene plasmid # 233898 ; http://n2t.net/addgene:233898 ; RRID:Addgene_233898)

  • For your References section:

    Cytosolic NADK is conditionally essential for folate-dependent nucleotide synthesis. Flickinger KM, Mellado Fritz CA, Huggler KS, Wade GM, Chang GR, Fox KC, Simcox JA, Cantor JR. Nat Metab. 2025 May 2. doi: 10.1038/s42255-025-01272-3. 10.1038/s42255-025-01272-3 PubMed 40316835
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