RMCE K>R DNA-PKcs
(Plasmid
#233917)
-
PurposeLow copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 233917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepsm7
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namehuman DNA-PKcs kinase inactive mutant K3753>R
-
SpeciesH. sapiens (human)
-
MutationK3753R
-
Entrez GenePRKDC (a.k.a. DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
RMCE K>R DNA-PKcs was a gift from Katheryn Meek (Addgene plasmid # 233917 ; http://n2t.net/addgene:233917 ; RRID:Addgene_233917) -
For your References section:
Two distinct long-range synaptic complexes promote different aspects of end processing prior to repair of DNA breaks by non-homologous end joining. Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, Chaplin AK, Meek K. Mol Cell. 2023 Mar 2;83(5):698-714.e4. doi: 10.1016/j.molcel.2023.01.012. Epub 2023 Jan 31. 10.1016/j.molcel.2023.01.012 PubMed 36724784