pAAV-syn-flex-iGluSnFR4s-NGR-WPRE
(Plasmid #234441)

• Purpose: AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
• Depositing Lab: GENIE Project
• Publication: Aggarwal et al bioRxiv 2025.03.20.643984

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: pAAV-Syn-FLEX
• Backbone manufacturer: Scott Sternson
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Depositing lab recommends limiting overnight liquid culture to 16 h at higher temperatures (37 C) to maintain ITR integrity, rather than 30 C for 20 h.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iGluSnFR4s-NGR
• Alt name: variant 8880
• Species: Synthetic; E. coli, A. victoria (jellyfish)
• Insert Size (bp): 1791
• Promoter: Synapsin
• Tags / Fusion Proteins:
  • IgK chain (N terminal on insert)
  • Myc epi tag-NGR GPI anchor (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pAAV-syn-flex-iGluSnFR4s-NGR-WPRE.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry