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PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV-Syn-FLEX
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Backbone manufacturerScott Sternson
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsDepositing lab recommends limiting overnight liquid culture to 16 h at higher temperatures (37 C) to maintain ITR integrity, rather than 30 C for 20 h.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiGluSnFR4s-NGR
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Alt namevariant 8880
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SpeciesSynthetic; E. coli, A. victoria (jellyfish)
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Insert Size (bp)1791
- Promoter Synapsin
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Tags
/ Fusion Proteins
- IgK chain (N terminal on insert)
- Myc epi tag-NGR GPI anchor (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-syn-flex-iGluSnFR4s-NGR-WPRE was a gift from GENIE Project (Addgene plasmid # 234441 ; http://n2t.net/addgene:234441 ; RRID:Addgene_234441) -
For your References section:
Glutamate indicators with increased sensitivity and tailored deactivation rates. Aggarwal A, Negrean A, Chen Y, Iyer R, Reep D, Liu A, Palutla A, Xie ME, MacLennan BJ, Hagihara KM, Kinsey LW, Sun JL, Yao P, Zheng J, Tsang A, Tsegaye G, Zhang Y, Patel RH, Arthur BJ, Hiblot J, Leippe P, Tarnawski M, Marvin JS, Vevea JD, Turaga SC, Tebo AG, Carandini M, Rossi LF, Kleinfeld D, Konnerth A, Svoboda K, Turner GC, Hasseman J, Podgorski K. bioRxiv 2025.03.20.643984 10.1101/2025.03.20.643984