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pET28b_H6-Smt3-AesC-GFP
(Plasmid #234486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone size w/o insert (bp) 5245
  • Total vector size (bp) 6445
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C-terminal Fragment of the Aes123 PolB1 Split Intein
  • Alt name
    AesC
  • Species
    Aeromonas phage Aes123
  • Insert Size (bp)
    1200
  • GenBank ID
    AFN69891.1 JN377899
  • Tags / Fusion Proteins
    • Ubiquitin-like protein Smt3 (N terminal on insert)
    • Hexahistidine Tag (N terminal on insert)
    • Superfolder Green Fluorescent Protein (GFP) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pET-T7side
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.22.634254 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b_H6-Smt3-AesC-GFP was a gift from Henning Mootz (Addgene plasmid # 234486 ; http://n2t.net/addgene:234486 ; RRID:Addgene_234486)

  • For your References section:

    A cysteine-less and ultra-fast split intein rationally engineered from being aggregation-prone to highly efficient in protein trans-splicing. Humberg C, Yilmaz Z, Fitzian K, Dorner W, Kummel D, Mootz HD. Nat Commun. 2025 Mar 19;16(1):2723. doi: 10.1038/s41467-025-57596-x. 10.1038/s41467-025-57596-x PubMed 40108172
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