pMAL-c2X_MBP-CLmN-H6
(Plasmid #234487)

• Purpose: Maltose binding protein N-terminally fused to His-tagged CLmN split intein
• Depositing Lab: Henning Mootz
• Publication: Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi: 10.1038/s41467-025-57596-x.

Backbone

• Vector backbone: pMAL-c2X
• Backbone size w/o insert (bp): 5445
• Total vector size (bp): 7020
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N-terminal Fragment of the CLm Split Intein
• Alt name: CLmN
• Species: Aeromonas phage Aes123
• Insert Size (bp): 360
• Mutation: T69K, F75H, M118N within the N-terminal fragment of the Aes123 PoB1 intein
• GenBank ID: AFN69891.1 JN377899
• Tags / Fusion Proteins:
  • Maltose Binding Protein (MBP) (N terminal on insert)
  • Hexahistidine Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: malE

Resource Information

• Supplemental Documents:
  • pCH145_MBP-IntN(T69K,F75H,M118N)-His6.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.22.634254 for bioRxiv preprint.