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pMAL-c2X_MBP-CLmN-H6
(Plasmid #234487)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-c2X
  • Backbone size w/o insert (bp) 5445
  • Total vector size (bp) 7020
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-terminal Fragment of the CLm Split Intein
  • Alt name
    CLmN
  • Species
    Aeromonas phage Aes123
  • Insert Size (bp)
    360
  • Mutation
    T69K, F75H, M118N within the N-terminal fragment of the Aes123 PoB1 intein
  • GenBank ID
    AFN69891.1 JN377899
  • Tags / Fusion Proteins
    • Maltose Binding Protein (MBP) (N terminal on insert)
    • Hexahistidine Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer malE
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.22.634254 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-c2X_MBP-CLmN-H6 was a gift from Henning Mootz (Addgene plasmid # 234487 ; http://n2t.net/addgene:234487 ; RRID:Addgene_234487)

  • For your References section:

    A cysteine-less and ultra-fast split intein rationally engineered from being aggregation-prone to highly efficient in protein trans-splicing. Humberg C, Yilmaz Z, Fitzian K, Dorner W, Kummel D, Mootz HD. Nat Commun. 2025 Mar 19;16(1):2723. doi: 10.1038/s41467-025-57596-x. 10.1038/s41467-025-57596-x PubMed 40108172
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