Ochre (rEcΔ2.ΔA.B3.tW*)
(Bacterial strain
#234622)
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PurposeGenomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Bacterial Strain | 234622 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
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Vector typeThis is a strain, not a plasmid
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Ochre (rEcΔ2.ΔA.B3.tW*)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMG1655.ΔTAG.ΔTGA.ΔprfA.mprfB3.A37G-tRNA-Trp
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Speciesn/a
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ochre (rEcΔ2.ΔA.B3.tW*) was a gift from Farren Isaacs (Addgene plasmid # 234622) -
For your References section:
Engineering a genomically recoded organism with one stop codon. Grome MW, Nguyen MTA, Moonan DW, Mohler K, Gurara K, Wang S, Hemez C, Stenton BJ, Cao Y, Radford F, Kornaj M, Patel J, Prome M, Rogulina S, Sozanski D, Tordoff J, Rinehart J, Isaacs FJ. Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08501-x. 10.1038/s41586-024-08501-x PubMed 39910296