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pUE001-Cas1:Dendra2Hfx
(Plasmid #234670)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234670 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUE001
  • Backbone size w/o insert (bp) 8075
  • Total vector size (bp) 9764
  • Vector type
    archeal expression in Haloferax volcanii
  • Selectable markers
    trpA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    growth in Haloferax volcannii: 42 - 45 °C growth temperature
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas1
  • Species
    Haloferax volcanii
  • Insert Size (bp)
    993
  • GenBank ID
    HVO_A0211
  • Promoter p.tna
  • Tag / Fusion Protein
    • Dendra2Hfx (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGTGAGCGAGGAAGCGGAAG
  • 3′ sequencing primer GTGGCGAGAAAGGAAGGGAAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a shuttle vector for replication in E.coli but protein expression and replication in the archaeon Haloferax volcanii

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUE001-Cas1:Dendra2Hfx was a gift from Ulrike Endesfelder (Addgene plasmid # 234670 ; http://n2t.net/addgene:234670 ; RRID:Addgene_234670)

  • For your References section:

    A novel expression system for imaging single-molecule fluorescence in Haloferax volcanii WR806 enables visualization of altered Cas1 dynamics during UV-induced DNA damage response. Schrage PR, Afonina U, Wörtz J, Marchfelder A, Martens KJA, Sáenz JP, Endesfelder U. μLife
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