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Addgene

p3HM2Luc
(Plasmid #234807)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234807 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Muscarinic M2 receptor
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Tags / Fusion Proteins
    • 3xHA (N terminal on insert)
    • RLuc8 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3HM2Luc was a gift from Vsevolod Gurevich (Addgene plasmid # 234807 ; http://n2t.net/addgene:234807 ; RRID:Addgene_234807)

  • For your References section:

    Manipulation of very few receptor discriminator residues greatly enhances receptor specificity of non-visual arrestins. Gimenez LE, Vishnivetskiy SA, Baameur F, Gurevich VV. J Biol Chem. 2012 Aug 24;287(35):29495-505. doi: 10.1074/jbc.M112.366674. Epub 2012 Jul 11. 10.1074/jbc.M112.366674 PubMed 22787152
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