pJPC596 FoMV-AmCyan
(Plasmid
#234812)
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PurposeTo express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnspecified
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAmCyan
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SpeciesAnemonia majano
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Amplified coding sequence of AmCyan along with FoMV duplicated coat protein promoter sequence insterted in using the oligos FoMV-AmCyan F-SapI and FoMV-AmCyan R-SapI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJPC596 FoMV-AmCyan was a gift from Daniel Voytas (Addgene plasmid # 234812 ; http://n2t.net/addgene:234812 ; RRID:Addgene_234812) -
For your References section:
Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery. Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF. Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 Dec 11. 10.1111/tpj.17196 PubMed 39661735