Thy1.1-G5
(Plasmid
#234839)
-
PurposepMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 234839 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMP71
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 6828
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameThy1.1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1332
-
Entrez GeneThy1 (a.k.a. CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2)
-
Tag
/ Fusion Protein
- EGFP-P2A-5xGly (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Thy1.1-G5 was a gift from Gabriel Victora (Addgene plasmid # 234839 ; http://n2t.net/addgene:234839 ; RRID:Addgene_234839) -
For your References section:
Universal recording of immune cell interactions in vivo. Nakandakari-Higa S, Walker S, Canesso MCC, van der Heide V, Chudnovskiy A, Kim DY, Jacobsen JT, Parsa R, Bilanovic J, Parigi SM, Fiedorczuk K, Fuchs E, Bilate AM, Pasqual G, Mucida D, Kamphorst AO, Pritykin Y, Victora GD. Nature. 2024 Mar;627(8003):399-406. doi: 10.1038/s41586-024-07134-4. Epub 2024 Mar 6. 10.1038/s41586-024-07134-4 PubMed 38448581