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pLV.tetO.Ascl1.U6.shRest1.U6.shRest2
(Plasmid #234846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Total vector size (bp) 9798
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ascl1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    696
  • Entrez Gene
    Ascl1 (a.k.a. ASH1, Mash1, bHLHa46)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV.tetO.Ascl1.U6.shRest1.U6.shRest2 was a gift from Malin Parmar (Addgene plasmid # 234846 ; http://n2t.net/addgene:234846 ; RRID:Addgene_234846)

  • For your References section:

    Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons. Nolbrant S, Giacomoni J, Hoban DB, Bruzelius A, Birtele M, Chandler-Militello D, Pereira M, Ottosson DR, Goldman SA, Parmar M. Stem Cell Reports. 2020 Oct 13;15(4):869-882. doi: 10.1016/j.stemcr.2020.08.013. Epub 2020 Sep 24. 10.1016/j.stemcr.2020.08.013 PubMed 32976765
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