pLenti-CMV-puro_FlnaABD-15-TS-16-24flag
(Plasmid #234869)

• Purpose: Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.
• Depositing Lab: Michael Murrell
• Publication: Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi: 10.1038/s41467-023-43612-5.

Backbone

• Vector backbone: pLenti-CMV-Puro
• Backbone size w/o insert (bp): 7971
• Total vector size (bp): 11610
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
• Species: H. sapiens (human)
• Insert Size (bp): 3682
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP, RFP, FLAG

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • pLENTI_CMV_Pro_Flna_ABD-15-TS-16-24.txt.gbk
• A portion of this plasmid was derived from a plasmid made by: Human FlnA tension sensor was a gift from Martin Schwartz. The tension sensor module was originally published in Kumar et al, 2016 (DOI: 10.1083/jcb.201510012)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry