pSIN-FHA2 R605A-Ypet-34-ECFP-hLaminA
(Plasmid
#235123)
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PurposeFHA2-mutated FRET biosensor R605A; FRET biosensor for live cell visualization of pSer22 Lamin A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepSin
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFHA2 R605A-Ypet-34-ECFP-hLaminA
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SpeciesH. sapiens (human)
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MutationFHA2 R605A
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIN-FHA2 R605A-Ypet-34-ECFP-hLaminA was a gift from Qin Peng (Addgene plasmid # 235123 ; http://n2t.net/addgene:235123 ; RRID:Addgene_235123) -
For your References section:
Genetically Encoded Fluorescence Resonance Energy Transfer Biosensor for Live-Cell Visualization of Lamin A Phosphorylation at Serine 22. Liu J, Li Q, Wang J, Qiu J, Zhou J, Peng Q. Biomater Res. 2024 Oct 22;28:0091. doi: 10.34133/bmr.0091. eCollection 2024. 10.34133/bmr.0091 PubMed 39440272