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Addgene

FLAG-GRP78-Wild-Type
(Plasmid #235169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235169 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9599
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    78-kDa glucose-regulated protein
  • Alt name
    GRP78
  • Alt name
    BiP
  • Alt name
    HSPA5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • GenBank ID
    NM_005347.5
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-GRP78-Wild-Type was a gift from Amy Shiu Lee (Addgene plasmid # 235169 ; http://n2t.net/addgene:235169 ; RRID:Addgene_235169)

  • For your References section:

    Cell surface relocalization of the endoplasmic reticulum chaperone and unfolded protein response regulator GRP78/BiP. Zhang Y, Liu R, Ni M, Gill P, Lee AS. J Biol Chem. 2010 May 14;285(20):15065-15075. doi: 10.1074/jbc.M109.087445. Epub 2010 Mar 5. 10.1074/jbc.M109.087445 PubMed 20208072
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