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Addgene

pLKO.1-puro-USP7-sh5
(Plasmid #235528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1-puro
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    USP7
  • gRNA/shRNA sequence
    CGTGGTGTCAAGGTGTACTAA
  • Species
    H. sapiens (human)
  • Entrez Gene
    USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1-puro-USP7-sh5 was a gift from Nicolas Manel (Addgene plasmid # 235528 ; http://n2t.net/addgene:235528 ; RRID:Addgene_235528)

  • For your References section:

    Centromeric DNA amplification triggered by viral proteins activates nuclear cGAS. Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig T, Dolken L, Lomonte P, Fachinetti D, Manel N. Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008. 10.1016/j.cell.2025.05.008 PubMed 40460826
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