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pPB_TRE3G_dCas9-5xGCN4_Hygro
(Plasmid #235572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235572 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB-TRE3G
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-5xGCN4
  • Species
    Other
  • Mutation
    D10A; H840A

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Drives DOX-inducible expression of the dCas9-5xGCN4 for epigenetic editing. This is a piggyBAC plasmid that integrates into the genome and can be selected for via Hygromycin resistance. An additional TET3G insert enables the doxycyline-indicuible system to operate.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB_TRE3G_dCas9-5xGCN4_Hygro was a gift from Jamie Hackett (Addgene plasmid # 235572 ; http://n2t.net/addgene:235572 ; RRID:Addgene_235572)

  • For your References section:

    Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications. Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA. Nat Genet. 2024 Jun;56(6):1168-1180. doi: 10.1038/s41588-024-01706-w. Epub 2024 May 9. 10.1038/s41588-024-01706-w PubMed 38724747
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