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Addgene

PECAM1-mRuby3-T2A-secNluc
(Plasmid #235607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositor does not recommend growing the plasmid in Kanamycin containing medium.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PECAM1 homologous recombination arms with PuroR drug selection cassette
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3523

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PECAM1-mRuby3-T2A-secNluc was a gift from Kai Wang (Addgene plasmid # 235607 ; http://n2t.net/addgene:235607 ; RRID:Addgene_235607)

  • For your References section:

    A novel quantitative angiogenesis assay based on visualized vascular organoid. Zhao Y, Sun M, Pan Z, Kong W, Hong Z, Zhang W, Sun B, Zhang J, Wang X, Wang K. Angiogenesis. 2025 Jan 3;28(1):10. doi: 10.1007/s10456-024-09967-z. 10.1007/s10456-024-09967-z PubMed 39751990
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