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pLenti PGK Blast LUC (w528-1)
(Plasmid #235669)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235669 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti PGK Blast V5-Luc(w528-1)
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman (Addgene plasmid # 19166)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Species
    firefly

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHi (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti PGK Blast LUC (w528-1) was a gift from Kevin Janes (Addgene plasmid # 235669 ; http://n2t.net/addgene:235669 ; RRID:Addgene_235669)

  • For your References section:

    PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com cells. Marohl T, Atkins KA, Wang L, Janes KA. Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211. 10.1158/1541-7786.MCR-25-0211 PubMed 41148168
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