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Addgene

pYO1031
(Plasmid #235706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab also recommends XL10-gold E. coli for transformation.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Beclin 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BECN1 (a.k.a. ATG6, VPS30, beclin1)
  • Promoter CMV

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    UVRAG
  • Species
    H. sapiens (human)
  • Entrez Gene
    UVRAG (a.k.a. DHTX, VPS38, p63)
  • Promoter CAG

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For other work using this plasmid, please see Buckles et al. 2020 PMID 33137306; Tremel et al. 2021 PMID 33692360.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO1031 was a gift from Roger Williams (Addgene plasmid # 235706 ; http://n2t.net/addgene:235706 ; RRID:Addgene_235706)

  • For your References section:

    Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes. Ohashi Y, Tremel S, Masson GR, McGinney L, Boulanger J, Rostislavleva K, Johnson CM, Niewczas I, Clark J, Williams RL. Elife. 2020 Jun 30;9:e58281. doi: 10.7554/eLife.58281. 10.7554/eLife.58281 PubMed 32602837
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