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Addgene

pcDNA3.1-SARS-CoV-2-S-ectodomain
(Plasmid #235984)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235984 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5402
  • Total vector size (bp) 9386
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Spike protein
  • Species
    SARS-CoV-2
  • Mutation
    aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987PP substitutions
  • GenBank ID
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tags / Fusion Proteins
    • internal Twin-Strep tag inserted between residues Leu18 and Thr19
    • 8xHis and Twin-Strep tag (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthesized by TOPGENE Inc.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-SARS-CoV-2-S-ectodomain was a gift from Syed Saif Hasan (Addgene plasmid # 235984 ; http://n2t.net/addgene:235984 ; RRID:Addgene_235984)

  • For your References section:

    Production and cryo-electron microscopy structure of an internally tagged SARS-CoV-2 spike ecto-domain construct. Singh S, Liu Y, Burke M, Rayaprolu V, Stein SE, Hasan SS. J Struct Biol X. 2025 Feb 11;11:100123. doi: 10.1016/j.yjsbx.2025.100123. eCollection 2025 Jun. 10.1016/j.yjsbx.2025.100123 PubMed 40046771
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