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pcDNA3-Lyso-INPP5E
(Plasmid #236004)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236004 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5403
  • Total vector size (bp) 8694
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lyso-INPP5E
  • Alt name
    LAMP1-INPP5E(214-644)-mCherry
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3291
  • Entrez Gene
    LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
  • Entrez Gene
    INPP5E (a.k.a. CORS1, CPD4, JBTS1, MORMS, PPI5PIV, pharbin)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Full-length LAMP1 (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Lyso-INPP5E was a gift from Jin Zhang (Addgene plasmid # 236004 ; http://n2t.net/addgene:236004 ; RRID:Addgene_236004)

  • For your References section:

    Lysosomal PIP(3) revealed by genetically encoded lipid biosensors. Sahan AZ, Chen M, Su Q, Li Q, Wang D, Zhang J. Proc Natl Acad Sci U S A. 2025 Apr;122(13):e2426929122. doi: 10.1073/pnas.2426929122. Epub 2025 Mar 24. 10.1073/pnas.2426929122 PubMed 40127277
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