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Addgene

pHE111-3xFlag-HSP70
(Plasmid #236055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236055 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p3xFLAG-CMV-10
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSP70
  • Alt name
    HSPA1A
  • Alt name
    heat shock protein family A (Hsp70) member 1A
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Entrez Gene
    HSPA1A (a.k.a. HEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHE111-3xFlag-HSP70 was a gift from Jonathan Schisler (Addgene plasmid # 236055 ; http://n2t.net/addgene:236055 ; RRID:Addgene_236055)

  • For your References section:

    Phosphorylation-State Modulated Binding of HSP70: Structural Insights and Compensatory Protein Engineering. Stewart M, Paththamperuma C, McCann C, Cottingim K, Zhang H, DelVecchio R, Peng I, Fennimore E, Nix JC, Saeed MN, George K, Makaroff M, Colie M, Paulakonis E, Almeida MF, Afolayan AJ, Brown NG, Page RC, Schisler JC. bioRxiv 2025.02.17.637997 10.1101/2025.02.17.637997
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