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pT/CAGGS-NRASG12V-IRES-mVenus
(Plasmid #236076)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236076 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT
  • Total vector size (bp) 7719
  • Vector type
    Sleeping beauty transposon plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mVenus
  • Species
    Aequorea victoria
  • Insert Size (bp)
    717
  • GenBank ID
    DQ092360
  • Promoter CAGGS

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    NRAS G12V
  • Species
    H. sapiens (human)
  • Mutation
    G12V
  • GenBank ID
    NG_007572
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Promoter CAGGS

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT/CAGGS-NRASG12V-IRES-mVenus was a gift from Masashi Narita (Addgene plasmid # 236076 ; http://n2t.net/addgene:236076 ; RRID:Addgene_236076)

  • For your References section:

    Titration of RAS alters senescent state and influences tumour initiation. Chan ASL, Zhu H, Narita M, Cassidy LD, Young ARJ, Bermejo-Rodriguez C, Janowska AT, Chen HC, Gough S, Oshimori N, Zender L, Aitken SJ, Hoare M, Narita M. Nature. 2024 Sep;633(8030):678-685. doi: 10.1038/s41586-024-07797-z. Epub 2024 Aug 7. 10.1038/s41586-024-07797-z PubMed 39112713
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