pcDNA3_mSGH-AKAR3EV
(Plasmid
#236207)
-
PurposeFluorescent biosensor for cytosolic PKA activity
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 236207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepcDNA3
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameQC2-AKAR-Halotag
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_FW (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3_mSGH-AKAR3EV was a gift from Peter Dedecker (Addgene plasmid # 236207 ; http://n2t.net/addgene:236207 ; RRID:Addgene_236207) -
For your References section:
Reduced Artifacts in Nanoscale Ratiometric Imaging and Sensing via Point-Spread Function Matching. Van Thillo T, Van Deuren V, Van den Eynde R, Vandenberg W, Dedecker P. ACS Nano. 2025 Jul 1;19(25):22755-22766. doi: 10.1021/acsnano.4c16338. Epub 2025 Jun 20. 10.1021/acsnano.4c16338 PubMed 40539535