Skip to main content
Addgene

pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE
(Plasmid #236242)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSIV third-generation, simian immunodeficiency based, pro-lentiviral vector
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4200
  • Promoter human Synapsin 1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site BsrGI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 236242 ; http://n2t.net/addgene:236242 ; RRID:Addgene_236242)

  • For your References section:

    Periodic ER-plasma membrane junctions support long-range Ca(2+) signal integration in dendrites. Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, Lippincott-Schwartz J. Cell. 2025 Jan 23;188(2):484-500.e22. doi: 10.1016/j.cell.2024.11.029. Epub 2024 Dec 20. 10.1016/j.cell.2024.11.029 PubMed 39708809
Related items: