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pLV-Hygro-TRE>{MCS}:T2A:EGFP
(Plasmid #236250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Mammalian Tet Inducible Gene Expression Lentiviral Vector
  • Backbone manufacturer
    VectorBuilder
  • Backbone size (bp) 6700
  • Modifications to backbone
    Added the coding sequence for a multicloning site with no stop codon directly downstream of the TRE sequence, linked to EGFP with a T2A sequence; added a hygromycin resistance gene for antibiotic selection
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter TRE
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-Hygro-TRE>{MCS}:T2A:EGFP was a gift from Genevieve Kendall (Addgene plasmid # 236250 ; http://n2t.net/addgene:236250 ; RRID:Addgene_236250)

  • For your References section:

    New dual inducible cellular model to investigate temporal control of oncogenic cooperating genes. Kent MR, Jay AN, Kendall GC. Sci Rep. 2024 Sep 5;14(1):20773. doi: 10.1038/s41598-024-71227-3. 10.1038/s41598-024-71227-3 PubMed 39237585
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