p3HAT1Luc1
(Plasmid
#236275)
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PurposeMammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8
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Depositing Lab
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Depositing OrganizationVanderbilt University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 236275-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 236275-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 4400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameangiotensin II type 1 receptor
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Alt nameclone p3HAT1Luc1
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SpeciesH. sapiens (human)
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Entrez GeneAGTR1 (a.k.a. AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, ATR1, HAT1R)
- Promoter CMV
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Tags
/ Fusion Proteins
- 3xHA (N terminal on insert)
- Rluc8 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco RI (unknown if destroyed)
- 3′ cloning site Hind III (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The MCS of the pcDNA3 vector was modified, so the structure of insert is: EcoRI - AsiSI - Start 3xHA-AT1R - SbfI - RLuc8 - Stop AscI - XbaI - PacI - HindIII
DNA (Catalog # 236275-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 236275-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3HAT1Luc1 was a gift from Vsevolod Gurevich (Addgene plasmid # 236275 ; http://n2t.net/addgene:236275 ; RRID:Addgene_236275) -
For your References section:
Manipulation of very few receptor discriminator residues greatly enhances receptor specificity of non-visual arrestins. Gimenez LE, Vishnivetskiy SA, Baameur F, Gurevich VV. J Biol Chem. 2012 Aug 24;287(35):29495-505. doi: 10.1074/jbc.M112.366674. Epub 2012 Jul 11. 10.1074/jbc.M112.366674 PubMed 22787152
