pcDNA_mIGg2a_Anti-HA [12CA5] v2
(Plasmid
#236295)
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PurposeMammalian vector expressing Anti-HA [12CA5] variable region fused to mouse IgG2a heavy chain
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Depositing Labs
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 6122
- Total vector size (bp) 7532
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAnti-HA [12CA5] heavy chain variable region fused to mouse IgG2a heavy chain
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SpeciesM. musculus (mouse)
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Insert Size (bp)1410
- Promoter CMV
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Tag
/ Fusion Protein
- Mouse IgG2a Fc (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA_mIGg2a_Anti-HA [12CA5] v2 was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 236295 ; http://n2t.net/addgene:236295 ; RRID:Addgene_236295)
Map uploaded by the depositor.
