pcDNA HA DUSP6
(Plasmid
#236430)
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Purposetransient overexpression of DUSP6 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDUSP6
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SpeciesH. sapiens (human)
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Entrez GeneDUSP6 (a.k.a. HH19, MKP3, PYST1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA HA DUSP6 was a gift from Michele Pagano (Addgene plasmid # 236430 ; http://n2t.net/addgene:236430 ; RRID:Addgene_236430) -
For your References section:
Loss of FBXO31-mediated degradation of DUSP6 dysregulates ERK and PI3K-AKT signaling and promotes prostate tumorigenesis. Duan S, Moro L, Qu R, Simoneschi D, Cho H, Jiang S, Zhao H, Chang Q, de Stanchina E, Arbini AA, Pagano M. Cell Rep. 2021 Oct 19;37(3):109870. doi: 10.1016/j.celrep.2021.109870. 10.1016/j.celrep.2021.109870 PubMed 34686346