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Addgene

pMEDS
(Plasmid #236568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMA-RQ
  • Backbone size w/o insert (bp) 1357
  • Total vector size (bp) 5683
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; cytosine deaminase/uracil phosphoribosyltransferase (select with 5-FC)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CD/UPRT (negative selection)
  • Alt name
    cytosine deaminase/uracil phosphoribosyltransferase (CD/UPRT)
  • Insert Size (bp)
    1125
  • Tag / Fusion Protein
    • 3x Myc

Gene/Insert 2

  • Gene/Insert name
    NPT II (positive selection)
  • Alt name
    Neomycin Phosphotransferase II
  • Insert Size (bp)
    792
  • Tag / Fusion Protein
    • FLAG

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMEDS was a gift from Shannon Moonah (Addgene plasmid # 236568 ; http://n2t.net/addgene:236568 ; RRID:Addgene_236568)

  • For your References section:

    CRISPR genome editing using a combined positive and negative selection system. Sharma I, Hall K, Moonah S. PLoS One. 2025 May 6;20(5):e0321881. doi: 10.1371/journal.pone.0321881. eCollection 2025. 10.1371/journal.pone.0321881 PubMed 40327602
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