pAAV-BEC enhancer-minBG-iCre(R279T)-4x6T
(Plasmid #236727)

• Purpose: Brain Endothelial Cell Enhancer with 4x6T miRNA targeting cassette For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.
• Depositing Lab: Xiangmin Xu
• Publication: Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6. doi: 10.1016/j.neuron.2025.03.031.

Backbone

• Vector backbone: pAAV2
• Backbone manufacturer: AAVnergene
• Backbone size w/o insert (bp): 2896
• Total vector size (bp): 5640
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: cis-regulatory elements 89763
• Alt name: cCREs89763
• Species: M. musculus (mouse)
• Insert Size (bp): 501
• GenBank ID: AL604023.12

Gene/Insert 2

• Gene/Insert name: iCre(R297T)
• Species: Synthetic
• Insert Size (bp): 1029
• Mutation: R297T

Gene/Insert 3

• Gene/Insert name: 4x6T miRNA targeting cassette
• Species: Synthetic
• Insert Size (bp): 529

Resource Information

• Supplemental Documents:
  • pAAV-BEC enhancer-minBG-iCRE(279T)-4x6T.gbk
• A portion of this plasmid was derived from a plasmid made by: iCre from Jonathan Ting, Allen Insitute (Addgene 164450); 4x6T from Stanley Thomas Carmichael (Addgene 196411)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry