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pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2
(Plasmid #236731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236731 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti_DualsgRNA_EmptyVector_Puro_T2A_TagBFP2
  • Backbone manufacturer
    Derek Narendra, Addgene plasmid #236729
  • Backbone size w/o insert (bp) 9585
  • Total vector size (bp) 9958
  • Modifications to backbone
    pLenti_DualsgRNA_EmptyVector_Puro_T2A_TagBFP2 digested with BsmBI and ligated with "TIMM23 fragment" via NEBuilder.
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TIMM23
  • gRNA/shRNA sequence
    GTTGAGGAGTAACGGCCCAG, GGAAGGTCAGCGTGTGAAGT
  • Species
    H. sapiens (human)
  • Entrez Gene
    TIMM23 (a.k.a. TIM23)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.19.639160 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2 was a gift from Derek Narendra (Addgene plasmid # 236731 ; http://n2t.net/addgene:236731 ; RRID:Addgene_236731)

  • For your References section:

    A unified mechanism for mitochondrial damage sensing in PINK1-Parkin-mediated mitophagy. Thayer JA, Petersen JD, Huang X, Gruel Budet LM, Hawrot J, Ramos DM, Sekine S, Li Y, Ward ME, Narendra DP. EMBO J. 2025 Nov 20. doi: 10.1038/s44318-025-00604-z. 10.1038/s44318-025-00604-z PubMed 41266657
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