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pET28a-TBD
(Plasmid #236737)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236737 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Thalidomide Binding domain of hCRBN
  • Species
    H. sapiens (human)
  • Entrez Gene
    CRBN (a.k.a. MRT2, MRT2A)
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-TBD was a gift from Christina Woo (Addgene plasmid # 236737 ; http://n2t.net/addgene:236737 ; RRID:Addgene_236737)

  • For your References section:

    A method for the detection and enrichment of endogenous cereblon substrates. Lloyd HC, Li Y, Payne NC, Zhao Z, Xu W, Kroupova A, Zollman D, Long T, Kabir F, Chen M, Freeman R, Feng EY, Xi SY, Hsu YC, Ciulli A, Mazitschek R, Woo CM. Cell Chem Biol. 2025 Aug 5:S2451-9456(25)00225-9. doi: 10.1016/j.chembiol.2025.07.002. 10.1016/j.chembiol.2025.07.002 PubMed 40782806
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