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Addgene

pET-28c-F30-2xdBroccoli-47CAG
(Plasmid #237217)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237217 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c-F30-2xdBroccoli
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5445
  • Total vector size (bp) 5637
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    47 CAG trinucleotide repeats RNA
  • Species
    Synthetic
  • Insert Size (bp)
    192

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ggtgatgtcggcgatatagg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28c-F30-2xdBroccoli-47CAG was a gift from Mingxu You (Addgene plasmid # 237217 ; http://n2t.net/addgene:237217 ; RRID:Addgene_237217)

  • For your References section:

    Targeted RNA condensation in living cells via genetically encodable triplet repeat tags. Xue Z, Ren K, Wu R, Sun Z, Zheng R, Tian Q, Ali AA, Mi L, You M. Nucleic Acids Res. 2023 Sep 8;51(16):8337-8347. doi: 10.1093/nar/gkad621. 10.1093/nar/gkad621 PubMed 37486784
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