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Addgene

pQE-80L-V4-ELP-EEP13
(Plasmid #237223)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237223 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pQE80-L
  • Backbone size w/o insert (bp) 4499
  • Total vector size (bp) 6758
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab uses use Top10f' for expansion and BL21*DE3 for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V4-ELP-EEP13
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE-80L-V4-ELP-EEP13 was a gift from Elliot Chaikof (Addgene plasmid # 237223 ; http://n2t.net/addgene:237223 ; RRID:Addgene_237223)
  • For your References section:

    Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins. Eweje F, Ibrahim V, Shajii A, Walsh ML, Ahmad K, Alrefai A, Miyasato D, Davis JR, Ham H, Li K, Roehrl M, Haller CA, Liu DR, Chen J, Chaikof EL. Nat Biotechnol. 2025 May 15. doi: 10.1038/s41587-025-02664-2. 10.1038/s41587-025-02664-2 PubMed 40374955