pLV-mCherry-Parkin
(Plasmid
#237397)
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PurposeLentivirus plasmid for the stable expression of mCherry-Parkin in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepSicoR
- Total vector size (bp) 9181
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Vector typeMammalian Expression, Lentiviral
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Selectable markersmCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePRKN
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SpeciesH. sapiens (human)
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Insert Size (bp)2133
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Entrez GenePRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
- Promoter EF1A
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BsrGI (destroyed during cloning)
- 5′ sequencing primer EF-1a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byRichard Youle
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.24.645001 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-mCherry-Parkin was a gift from James Hurley (Addgene plasmid # 237397 ; http://n2t.net/addgene:237397 ; RRID:Addgene_237397) -
For your References section:
In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment. Rose K, Herrmann E, Kakudji E, Lizarrondo J, Celebi AY, Wilfling F, Lewis SC, Hurley JH. Proc. Natl. Acad. Sci. U.S.A., 122(31), e2511890122 10.1073/pnas.2511890122