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Addgene

pLV-mCherry-Parkin
(Plasmid #237397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237397 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSicoR
  • Total vector size (bp) 9181
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRKN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2133
  • Entrez Gene
    PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
  • Promoter EF1A
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BsrGI (destroyed during cloning)
  • 5′ sequencing primer EF-1a
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Richard Youle

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.24.645001 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-mCherry-Parkin was a gift from James Hurley (Addgene plasmid # 237397 ; http://n2t.net/addgene:237397 ; RRID:Addgene_237397)

  • For your References section:

    In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment. Rose K, Herrmann E, Kakudji E, Lizarrondo J, Celebi AY, Wilfling F, Lewis SC, Hurley JH. Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2511890122. doi: 10.1073/pnas.2511890122. Epub 2025 Jul 31. 10.1073/pnas.2511890122 PubMed 40743392
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