pLV-BFP-Mito
(Plasmid
#237398)
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PurposeLentivirus plasmid for the stable expression of mitochondrial matrix-localized BFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepSicoR
- Total vector size (bp) 7859
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Vector typeMammalian Expression, Lentiviral
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Selectable markersEBFP2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOX8A
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Alt nameCOX8A
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SpeciesH. sapiens (human)
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Insert Size (bp)828
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Entrez GeneCOX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
- Promoter EF1A
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Tag
/ Fusion Protein
- EBFP2 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer EF-1a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMichael Davidson
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.24.645001 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-BFP-Mito was a gift from James Hurley (Addgene plasmid # 237398 ; http://n2t.net/addgene:237398 ; RRID:Addgene_237398) -
For your References section:
In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment. Rose K, Herrmann E, Kakudji E, Lizarrondo J, Celebi AY, Wilfling F, Lewis SC, Hurley JH. Proc. Natl. Acad. Sci. U.S.A., 122(31), e2511890122 10.1073/pnas.2511890122