pKOS6b-URP114
(Plasmid
#237505)
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PurposeUpregulation of mgdh with P114
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepKOS6b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemembrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
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Alt nameGene ID: 56906566
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Alt namemgdh
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SpeciesGluconobacter oxydans
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MutationUpregulation of mgdh with P114
Resource Information
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A portion of this plasmid was derived from a plasmid made byAlexa M. Schmitz.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.02.09.527855 for bioRxiv preprint.
Addgene observed 297 base pair deletion downstream of M13 rev and 300bp deletion upstream of M13 fwd. The depositor has confirmed that this was not of concern to plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKOS6b-URP114 was a gift from Buz Barstow (Addgene plasmid # 237505 ; http://n2t.net/addgene:237505 ; RRID:Addgene_237505)