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Addgene

pKOS6b-URP114
(Plasmid #237505)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237505 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pKOS6b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
  • Alt name
    Gene ID: 56906566
  • Alt name
    mgdh
  • Species
    Gluconobacter oxydans
  • Mutation
    Upregulation of mgdh with P114

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Alexa M. Schmitz.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.02.09.527855 for bioRxiv preprint.
Addgene observed 297 base pair deletion downstream of M13 rev and 300bp deletion upstream of M13 fwd. The depositor has confirmed that this was not of concern to plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKOS6b-URP114 was a gift from Buz Barstow (Addgene plasmid # 237505 ; http://n2t.net/addgene:237505 ; RRID:Addgene_237505)