pORTMAGE-Cc1
(Plasmid
#237798)
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PurposeIntegrating SSAP (HapRecT) and cutting cas9 (Sth3) into Caulobacter crescentus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXGFPC-5
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Vector typeBacterial Expression, CRISPR ; Donor vector for Caulobacter crescentus
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameHapRecT
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SpeciesHafnia alvei
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Insert Size (bp)786
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GenBank IDWP_004092500
- Promoter pXYL promoter
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameSth3 Cas9
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Insert Size (bp)4167
- Promoter pXYL promoter
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pORTMAGE-Cc1 was a gift from Timothy Wannier (Addgene plasmid # 237798)