pIVT-Cas9-TMV
(Plasmid #238016)

• Purpose: for DNA-free gene knockout editing in rice and wheat or other plants
• Depositing Lab: Caixia Gao
• Publication: Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-1358. doi: 10.1111/pbi.14591. Epub 2025 Feb 10.

Backbone

• Vector backbone: pnCas9-PBE
• Backbone manufacturer: Caixia Gao, Addgene Plasmid #98164
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Growth instructions: While the plasmid can be maintained in DH5alpha, please note that the polyA sequence on the plasmid may experience partial adenine (A) loss during E. coli amplification. Therefore, it's essential to pick single colonies for sequencing verification after expansion. The depositing lab specifically selected the XL10 strain because it demonstrates relatively less polyA sequence loss compared to other strains.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpCas9
• Species: Other
• Promoter: T7

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pIVT-Cas9-TMV.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry