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pME234
(Plasmid #238104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238104 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV
  • Total vector size (bp) 9024
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TadA*7.10(Y123H/N157K)-m_SpnCas9(D10A)
  • Alt name
    ABE7.10-HK NG-nCas9
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    5625
  • Mutation
    TadA modifications include Y123H, N157K; NG-nCas9 modifications include D10A (nick) and R1335V, L1111R, D1135V, G1218R, E1219F, A1322R, T1337R (NG)
  • Tags / Fusion Proteins
    • SV40 bpNLS-P2A-mCherry (C terminal on insert)
    • SV40 bpNLS (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME234 was a gift from Alexis Komor (Addgene plasmid # 238104 ; http://n2t.net/addgene:238104 ; RRID:Addgene_238104)
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