pLX_305_OVA_Blasticidin
(Plasmid
#238124)
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PurposeEnables expression of Ovalbumin with Blasticidin selectable marker.
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Depositing Lab
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Depositing OrganizationHarvard Medical School
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 238124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 238124-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 238124-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepLX_305
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Backbone manufacturerBroad Institute Genetic Perturbation Platform
- Backbone size w/o insert (bp) 7408
- Total vector size (bp) 8569
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOvalbumin
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SpeciesG. gallus (chicken)
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Insert Size (bp)1161
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GenBank ID396058
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Entrez GeneOVAL (a.k.a. OVA, SERPINB14)
- Promoter Human PGK
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CAATAGCGGCTGCTCAGCA
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGenscript performed this cloning.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 238124-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 238124-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX_305_OVA_Blasticidin was a gift from Arlene Sharpe (Addgene plasmid # 238124 ; http://n2t.net/addgene:238124 ; RRID:Addgene_238124) -
For your References section:
A STUB1-CHIC2 complex inhibits CD8(+) T cells to restrain tumor immunity. LaFleur MW, Milling LE, Prathima P, Li V, Lemmen AM, Streeter ISL, Heisig PKS, Derosia NM, Riffo E, Xu H, Nguyen TH, Kolengaden A, Markson SC, Doench JG, Sharpe AH. Nat Immunol. 2025 Sep;26(9):1476-1487. doi: 10.1038/s41590-025-02231-6. Epub 2025 Aug 12. 10.1038/s41590-025-02231-6 PubMed 40796662
Map uploaded by the depositor.
