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pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)
(Plasmid #238185)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238185 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRCA847
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KLF4
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Tag / Fusion Protein
    • mTagBFP2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.31.606073 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone) was a gift from Thomas Norman (Addgene plasmid # 238185 ; http://n2t.net/addgene:238185 ; RRID:Addgene_238185)

  • For your References section:

    Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. 10.1101/2024.07.31.606073 PubMed 39131349
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