pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)
(Plasmid
#238185)
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PurposeLentiviral vector for overexpression KLF4 ORF from a EF1a promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRCA847
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKLF4
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SpeciesH. sapiens (human), Synthetic
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Entrez GeneKLF4 (a.k.a. EZF, GKLF)
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Tag
/ Fusion Protein
- mTagBFP2 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.31.606073 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone) was a gift from Thomas Norman (Addgene plasmid # 238185 ; http://n2t.net/addgene:238185 ; RRID:Addgene_238185) -
For your References section:
Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, Guruvayurappan K, Norman TM. bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 10.1101/2024.07.31.606073. 10.1101/2024.07.31.606073 PubMed 39131349