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pCMV-NLS-dCas13-NLS-eNAT10
(Plasmid #238300)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238300 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EBFP2-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dPspCas13b fused to eNAT10
  • Alt name
    N-acetyltransferase 10
  • Species
    H. sapiens (human); Prevotella sp. P5-125
  • Mutation
    H133A for catalytically inactive mutant, deletion of aa984-1090 in PspCas13b; deletion of aa802-1025 from hNAT10
  • Entrez Gene
    NAT10 (a.k.a. ALP, Kre33, NET43)
  • Tags / Fusion Proteins
    • SV40 BPNLS (N terminal on insert)
    • SV40 BPNLS (C terminal of dPspCas13b)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-NLS-dCas13-NLS-eNAT10 was a gift from Won Do Heo (Addgene plasmid # 238300 ; http://n2t.net/addgene:238300 ; RRID:Addgene_238300)

  • For your References section:

    Programmable RNA acetylation with CRISPR-Cas13. Yu J, Jin J, Kwon E, Jang H, Choi SK, Kim D, Kim C, Son S, Yoon KJ, Heo WD. Nat Chem Biol. 2025 Jun 2. doi: 10.1038/s41589-025-01922-3. 10.1038/s41589-025-01922-3 PubMed 40456962
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