pTol2-cmlc2-nGFP-nCas9
(Plasmid #238308)

• Purpose: Expression of nuclear GFP and nuclear Cas9 specifically in cardiomyocytes driven by the cmlc2(myl7) promoter.
• Depositing Lab: Juan Manuel González-Rosa
• Publication: Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):101003. doi: 10.1016/j.crmeth.2025.101003.

Backbone

• Vector backbone: pTol2 backbone
• Total vector size (bp): 10016
• Vector type: Tol2-based plasmid

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9 (Csn1) endonuclease from Streptococcus pyogenes
• Alt name: Cas9
• Species: D. rerio (zebrafish); Streptococcus pyogenes
• Insert Size (bp): 4101
• Promoter: cmlc2 (myl7)
• Tag / Fusion Protein:
  • nGFP-P2A

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pTol2-cmlc2-nGFP-nCas9n.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry